Mouse Anti-CRTAM Recombinant Antibody (clone 17B2)
CAT#: HPAB-1089-FY
This product is a recombinant antibody that recognizes CRTAM. The monoclonal antibody 17B2 reacts with the epitope of CRTAM.
Gene Expression
Tested Data
RNA Expression
Figure 1 RNA cell line category: Cell line enriched (SCLC-21H)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000109943-CRTAM
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SDS-PAGE
Figure 1 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in SDS-PAGE
SDS-PAGE analysis of HPAB-1089-FY in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in SEC-HPLC
The purity of HPAB-1089-FY was greater than 98% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
DB
Figure 3 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in DB
Dot Blot analysis of HPAB-1089-FY was performed by coating with human CRTAM protein (His tag).
HPAB-1089-FY incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)
WB
Figure 4 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in WB
Western blot analysis of HPAB-1089-FY was performed by loading human CRTAM protein, His tag.
HPAB-1089-FY incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L).
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 2: Reducing Antigen (0.6 μg)
ELISA
Figure 5 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in ELISA
ELISA analysis of HPAB-1089-FY was performed by coating with human CRTAM protein (His tag). Then blocked with BSA and incubated with HPAB-1089-FY. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Host Species
- Mouse
- Derivation
- Chimeric (hamster/mouse)
- Type
- Mouse IgG
- Specificity
- Human CRTAM
- Species Reactivity
- Human
- Clone
- 17B2
- Applications
- WB, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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High Batch Consistency
We've ordered multiple batches of this antibody, and each batch has been consistent in quality and performance.
Good Value
Considering the high quality and performance, this antibody is very cost-effective. Great value for the price.
Perfect for Research
This is an ideal research antibody with proven reliability in our lab. It meets all our research needs effectively.
Q&As
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Do antibodies need to be refrigerated?
A: Many antibodies require refrigeration to maintain their stability and effectiveness. Always follow the manufacturer's storage instructions.
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What is the lifespan of an antibody?
A: The shelf life of antibody products varies from a few months to many years, depending on the formulation and storage conditions.
-
How are antibodies preserved?
A: To keep antibodies effective, preserve them according to the manufacturer's instructions, typically in the refrigerator at a specific temperature.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-1089-FY, RRID: AB_3111415)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 17B2"
- CAT
- Product Name
See other products for "CRTAM"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2006MZ | Recombinant Mouse Anti-Human CRTAM Antibody (clone NN0305-32B0) | FC, WB | Mouse antibody |
| VS-0723-WK71 | Human Anti-CRTAM Recombinant Antibody (VS-0723-WK71) | FC | Chimeric (rabbit/human) IgG1 |
| VS-0723-WK81 | Human Anti-CRTAM Recombinant Antibody (VS-0723-WK81) | FC | Chimeric (rabbit/human) IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0811 | Hi-Affi™ Rabbit Anti-CRTAM Recombinant Antibody (clone DS811AB) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1089-FY-F(E) | Hamster Anti-CRTAM Recombinant Antibody (clone 17B2); scFv Fragment | WB, FC | Hamster scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1089-FY-S(P) | Mouse Anti-CRTAM Recombinant Antibody (clone 17B2); Fab Fragment | WB, FC | Chimeric(hamster/mouse) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY91 | CytoStream™ Mouse Anti-CRTAM Recombinant Antibody (VS-0225-XY91) | FC | Mouse IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC527 | Recombinant Anti-CRTAM Vesicular Antibody, EV Displayed (VS-0425-YC527) | ELISA, FC, Cell-uptake |
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